Bright cyan fluorescent protein variants identified by fluorescence lifetime screening
| Authors |
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| Publication date | 2010 |
| Journal | Nature Methods |
| Volume | Issue number | 7 | 2 |
| Pages (from-to) | 137-139 |
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| Abstract | Optimization of autofluorescent proteins by intensity-based screening of bacteria does not necessarily identify the brightest variant for eukaryotes. We report a strategy to screen excited state lifetimes, which identified cyan fluorescent proteins with long fluorescence lifetimes (>3.7 ns) and high quantum yields (>0.8). One variant, mTurquoise, was 1.5-fold brighter than mCerulean in mammalian cells and decayed mono-exponentially, making it an excellent fluorescence resonance energy transfer (FRET) donor. |
| Document type | Article |
| Language | English |
| Published at |
https://doi.org/10.1038/NMETH.1415
(Final published version)
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