The catalytic machinery of hydrogenases.

Thesis

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Titlepage

CONTENTS

ABBREVIATIONS

I. GENERAL INTRODUCTION

II. INITIAL EVENTS DURING THE REACTION OF A {NIFE}-HYDROGENASE WITH HYDROGEN AS STUDIED BY FAST INFRARED SPECTROSCOPY

III. STOPPED-FLOW INFRARED STUDIES ON THE INTERRELATION BETWEEN THE ACTIVE STATES OF THE MEMBRANE-BOUND {NIFE}-HYDROGENASE FROM ALLOCHROMATIUM VINOSUM

IV. THE MEMBRANE-BOUND {NIFE}-HYDROGENASE (ECH) FROM METHANOSARCINA BARKERI: UNUSUAL PROPERTIES OF THE IRON-SULPHUR CLUSTERS

V. INITIAL SPECTROSCOPIC CHARACTERIZATION OF THE ACTIVE SITE IN THE MEMBRANE-BOUND {NIFE}-HYDROGENASE (ECH) FROM METHANOSARCINA BARKERI

VI. SUMMARY

Samenvatting

REFERENCES

ACKNOWLEDGMENTS

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