Fluorescence correlation spectroscopy
| Authors | |
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| Publication date | 2015 |
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| Book title | Advanced Fluorescence Microscopy: Methods and Protocols |
| ISBN |
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| Series | Methods in Molecular Biology, 1251 |
| Pages (from-to) | 135-150 |
| Number of pages | 294 |
| Publisher | New York: Humana Press |
| Organisations |
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| Abstract | Fluorescence fluctuation spectroscopy techniques allow the quantification of fluorescent molecules present at the nanomolar concentration level. After a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecular interaction of two signaling proteins inside the living cell using fluorescence cross-correlation spectroscopy. |
| Document type | Chapter |
| Language | English |
| Published at |
https://doi.org/10.1007/978-1-4939-2080-8_8
(Final published version)
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