Using genetically encoded fluorescent reporters to image lipid signalling in living plants

Authors
Publication date 2013
Host editors
  • T. Munnik
  • I. Heilmann
Book title Plant lipid signaling protocols
ISBN
  • 9781627034005
  • 9781493959273
ISBN (electronic)
  • 9781627034012
Series Methods in Molecular Biology
Pages (from-to) 283-289
Publisher New York: Humana Press
Organisations
  • Faculty of Science (FNWI) - Swammerdam Institute for Life Sciences (SILS)
Abstract The discovery of the green fluorescent protein has revolutionized cell biology as it allowed researchers to visualize dynamic processes in living cells. The fusion of fluorescent protein variants with lipid binding domains that bind to specific phospholipids have been very instrumental in investigating the role of these molecules in living plants. Here, we describe the use of these reporters to image lipids in living Arabidopsis seedlings using fluorescence microscopy.
Document type Chapter
Language English
Published at https://doi.org/10.1007/978-1-62703-401-2_26
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