Visualization of the moonlighting protein CD26DPPIV

Authors	E. Boonacker
Supervisors	C.J.F. van Noorden
Award date	18-06-2003
Number of pages	128
Organisations	Faculty of Medicine (AMC-UvA)
Document type	PhD thesis
Note	Research conducted at: CLDO
Language	English
Downloads	Thesis Cover Titlepage Contents CHAPTER 1 General introduction CHAPTER 2 Enzyme cytochemical techniques for metabolic mapping in living cells, with special reference to proteolysis CHAPTER 3 Fluorogenic substrate Ala-Pro-cresyl violet but not Ala-Pro-rhodamine 110 is cleaved specifically by DPPIV activity. A study in living Jurkat cells and CD26/DPPIV-transfected Jurkat cells CHAPTER 4 Determination of reactions of enzymes and their kinetic parameters in living cells by flow cytometry CHAPTER 5 Ala-Pro-cresyl violet, a synthetic fluorogenic substrate for the analysis of kinetic parameters of dipeptidyl peptidase IV (CD26) in individual living rat hepatocytes CHAPTER 6 CD26/DPPIV signal transduction function, but not proteolytic activity, is directly related to its expression level on human Th1 and Th2 cell lines as detected with living cell cytochemistry CHAPTER 7 Rapid assay to detect natural protease substrates in living cells CHAPTER 8 The multifunctional or moonlighting protein CD26/DPPIV CHAPTER 9 Conclusions CHAPTER 10 Summary Samenvatting Dankwoord Curriculum Vitae Color illustrations Cover
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