Mechanistic studies on the diiron center in bovine spleen purple acid phosphatase

Authors	M. Merkx
Supervisors	B.A. Averill
Award date	11-05-1999
Organisations	Faculty of Science (FNWI) - Swammerdam Institute for Life Sciences (SILS)
Document type	PhD thesis
Note	Research conducted at: ECSI
Downloads	Thesis Cover Titlepage Contents Abbreviations Chapter 1: Introduction Chapter 2: Ga3+ as afunctional substitute of Fe3+: preparation and characterization of the Ga3+Fe2+ and Ga3+Zn2+ forms of bovine spleen purple acid phosphatase Chapter 3: Probing the role of the trivalent metal in phosphate ester hydrolysis: preparation and characterization of purple acid phosphatases containing Al(III)Zn(II) and In(III)Zn(II) active sites including the first example of an aluminum enzyme Chapter 4: The activity of oxidized bovine spleen purple acid phosphatase is due to an Fe(III)Zn(II)- 'impurity' Chapter 5: Evidence for non-bridged coordination of p-nitrophenylphosphate to the dinuclear Fe(III)-M(II) center in bovine spleen purple acid phosphatase during enzymatic turn-over Chapter 6: Fluoride inhibition of bovine spleen purple acid phosphatase: characterization of a ternary enzyme-phosphate-fluoride complex as a model for the active enzyme-substrate-hydroxide complex Chapter 7: Summary and concluding remarks Samenvatting Dankwoord Curriculum Vitae Cover
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