Immersion oil for high-resolution live-cell imaging at 37°C: Optical and physical characteristics
| Authors |
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|---|---|
| Publication date | 2008 |
| Journal | Journal of Microscopy |
| Volume | Issue number | 232 | 2 |
| Pages (from-to) | 353-361 |
| Organisations |
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| Abstract | The use of normal immersion oil, developed for 23°C, at 37°C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live-cell imaging at 37°C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23°C counterparts. |
| Document type | Article |
| Language | English |
| Published at |
https://doi.org/10.1111/j.1365-2818.2008.02106.x
(Final published version)
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