Lipid affinity beads: from identifying new lipid binding proteins to assessing their binding properties
| Authors | |
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| Publication date | 2013 |
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| Book title | Plant lipid signaling protocols |
| ISBN |
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| ISBN (electronic) |
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| Series | Methods in Molecular Biology |
| Pages (from-to) | 273-280 |
| Publisher | New York: Humana Press |
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| Abstract | Lipid affinity beads can be used to identify novel proteins with lipid binding capacity or to determine binding prerequisites of known lipid-binding proteins. Here we describe several applications for which this tool can be used and which considerations have to be taken into account. In addition to a precise protocol, several suggestions are made for experimental setups to facilitate identification of in vivo lipid binding targets. |
| Document type | Chapter |
| Language | English |
| Published at |
https://doi.org/10.1007/978-1-62703-401-2_25
(Final published version)
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