Less is more in herbarium-inclusive molecular ecology

This dataset relates to the manuscript entitled "Less is more in herbarium-inclusive molecular ecology: Universal kits capture considerable intraspecific variation", available as a pre-print at BioRXiv: ... The study describes a critical comparison between customised and universal approaches of target capture sequencing in herbarium genomics below the species level. A target capture sequencing tool (Hyb-Seq) was designed for genotyping apomictic clonal lineages of dandelions (Taraxacum officinale), where loci from three approaches were included: A) loci from a previous genotyping-by-sequencing (GBS) study on Taraxacum; B) genes custom selected based on evolutionary adaptive potential; C) loci from universal kits (Angiosperms-353 and Compositae-1061). The genotyping accuracy of these different sets of loci was tested on a set of five apomictic clonal lineages (ACLs) of Taraxacum officinale, including several members with the same multi-locus microsatellite genotype. This dataset comprises important background information to the accessions used in the study, the design of the target capture sequencing tool, scripts and bioinformatic pipelines used in the design of the tool and the analysis of the pilot dataset, and detailed results that were used in the production of the manuscript.