Isolation of primary avian osteocytes

Authors
Publication date 2012
Host editors
  • M.H. Helfrich
  • S.H. Ralston
Book title Bone Research Protocols
ISBN
  • 9781617794148
  • 9781493961009
ISBN (electronic)
  • 9781617794155
Series Methods in Molecular Biology
Edition 2nd
Pages (from-to) 43-53
Publisher New York: Humana Press
Organisations
  • Faculty of Dentistry (ACTA)
Abstract Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3.
Document type Chapter
Language English
Published at https://doi.org/10.1007/978-1-61779-415-5_4
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