Isolation of primary avian osteocytes
| Authors | |
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| Publication date | 2012 |
| Host editors |
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| Book title | Bone Research Protocols |
| ISBN |
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| ISBN (electronic) |
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| Series | Methods in Molecular Biology |
| Edition | 2nd |
| Pages (from-to) | 43-53 |
| Publisher | New York: Humana Press |
| Organisations |
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| Abstract | Osteocytes can be isolated from chicken calvaria using mild EDTA treatment alternating with collagenase treatment. The cell population obtained contains both osteoblasts and osteocytes. A pure population of osteocytes is obtained following immunomagnetic separation with the osteocyte-specific monoclonal antibody MAb OB7.3. |
| Document type | Chapter |
| Language | English |
| Published at |
https://doi.org/10.1007/978-1-61779-415-5_4
(Final published version)
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