Optimization of fluorescent proteins for novel quantitative multiparameter microscopy approaches

Authors	G.J. Kremers
Supervisors	T.W.J. Gadella (jr.)
Cosupervisors	J. Goedhart
Award date	25-10-2006
ISBN	9090208046 9789090208046
Number of pages	160
Organisations	Faculty of Science (FNWI) - Swammerdam Institute for Life Sciences (SILS)
Document type	PhD thesis
Note	Research conducted at: Universiteit van Amsterdam
Language	English
Downloads	Thesis Front cover Title pages Contents Chapter 1: Introduction Chapter 2: Cyan and yellow super fluorescent proteins with improved brightness, protein folding and FRET Förster radius Chapter 3: Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells Chapter 4: Fluorescence resonance energy transfer (FRET) measurement by gradual acceptor photobleaching Chapter 5: Quantitative lifetime unmixing of mixtures of two multiexponential decaying fluorescent species using single-frequency FLIM : application to multiparameter imaging of spectral identical fluorescent proteins Chapter 6: Summarizing discussion Abbreviations Color figures Samenvatting Curriculum vitae List of publications Back cover
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